Confocal Microscopy

Confocal Microscopy

Core facility has a Zeiss LSM710 confocal microscope, this can be used to

• analyze alive and fixed cells, there is a CO2- and temperature-controlled chamber for live cell imaging.
• detect your signal in 34 different channels across the wide wavelength range.
• do z-stacks and time-lapse, including multi-position time-lapse.
• observe cells in transmitting ligth

Technical parameters

• 4 lasers: 405 nm, 458/488/514 nm, 561 nm and 633 nm
• 4 objectives: 10 x, 20 x, 63 x (water, glycerol), 63 x (oil)

How to use a microscope

• Book your time in a calendar. If you change your plans, please change the booking as well.
• Before the first time you have to receive a proper training, please contact Reet Kurg (reet.kurg@ut.ee).
• Follow the instructions when using a microscope. When in doubt, please ask Eve Toomsoo (eve.toomsoo@ut.ee) to help you.
• When finished, please sign up in logbook just next to the microscope.